Research Class Report
Professor: Dr. Johnson
Research under Dr. X. Wang

Tuesday Sept. 29th 2009
First day of research Dr. Wang explained his work to us. Testing lean chicken DNA to DNA from a fat chicken, by figure out the differences in DNA. Wanting to find a gap or missing area of the DNA.

Thursday, Oct. 1st 2009
First I worked on the Nano drop ND-1000 SpectrophotometerˇK.I got the results on the amount of DNA from blood extracted from Chicken blood.
We saw how a PCR was run in the lab by the lab assistants.

Tuesday Oct. 6th 2009
Lab Asst TK Fester and I searched for a code to order new primers. Using primer express 2.0 softwareˇKwe pasted a sequence we were told to look for by Dr. Wang and designed primersˇKWe set to software mRNAˇKWhen we found the right 2 codes, we sent primer code off to a lab named Invirogen Illumina

Thursday Oct. 8th 2009
Today I worked on a protein concentrations with Dr. Wang and lab asst. Emenike. Showed how to calculate protein concentrations using the Nanodrop, after doing a western blot and electrolysis jell.

Tuesday Oct 13th 2009
The primers we ordered last week came inˇKWe started mixing the solution in a well plate to run a PCR on the primersˇKAt the end of this day we finished by placing the well plate to be ran in the computer which takes a few hours to run the PCR.

Thursday Oct 15th 2009
Today we extracted DNA from Chicken bloodˇKI wrote all the steps down in my lab book.

Tuesday Oct 20th 2009
Today I did my first PCR and Nanospec drop by myselfˇKI posted the results in my lab note bookˇKOf my 4 samples my 4th sample had the lowest amount of DNA that was isolated from the blood.


Thursday Oct 22nd 2009
We had a meeting today at 10:00 to go over the different research procedures, and see what direction we may go into with the microrayˇK.Today I extracted DNA from Chicken blood, and ran a Nanospec test to see how much DNA I have extracted.



Tuesday Nov. 3rd 2009
Today I went over Microarry software to see how it worksˇKand studied past research done on evaluation of custom microarray applicationsˇKas well as the oligoncleotide design challenge, and a paper on optimized design and assessment of whole genome tiliing arrays. The overall idea of this is to:
„H Identification of differentially expressed genes in the extreme fat chickens and extreme lean chickens
„R Isolate total RNA
„R Reverse transcription and fluorescent labeling
„R Microarray hybridization
„R Data processing and analysis


Thursday Nov. 5th 2009
We had a research meeting with Dr. Wang. We went over our past research experimentsˇKand related science papersˇKtitles areˇKTSPI copy Number Variation Influences Spermatogenesis and shows differences among Y Lineages
We also ran a jell electrophoresis on the DNA samples.

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Edited by author 08-16-2009 11:29 PM
1. You're the Captain of My Sea
2. Sing to Me
3. Call His Name
4. That's Why I Love You
5. Life
6. Signs of God
7. God's Sight
8. Time is Tickin' Away
9. Praise Jesus
10. Follow Me
11. Time to Fly
12. Come Into the Light
13. Calling All God's Children
14. I Can See Clearly Now
15. I Want My Angel Wings
16. Gensis, His 7 Days and 7 Nights
17. Lord Help Me Fix Up My Place
18. Searching Needing you

Sing to me
A melody
With bright green eyes
You’ll change my life
If you just sing to me

Sing to me
A mystery
We’ll have it all
And I just fall
If you sing to me

Sing to me
Sing to me
Sing to me

Sing to me
Alone with me
You’re outta sight
You rock my life
When you sing to me

Sing to me
Of what could be
You’ll be my star
and take me far
If you just sing to me

Sing to me
Sing to me
Sing to me

Sing songs of love and hate
Sing songs of passion and fate

Sing to me
Sing to me
Sing to me

Sing to me
A melody
We’ll have it all
And I just fall
If you sing to me

Sing to me
Sing to me
Sing to me
Sing to me
Sing to me
Sing

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Edited by author 04-24-2009 10:25 AM

BMI
609173

I was thirsty and could not see
That you were offering me something
That could quench my thirsting soul
Something that I needed to know
You’ve got water in a living cup
You telling me I should drink up
My little mind must comprehend
The blessing that you’re passing

Taste and See
Drink be free
Blood of Jesus

I was hungry for something more
Than what’s in this earth world
You had some bread I was told
The bread of life for my soul
You said if I just took a bite
My soul would be set right
Hand and hand I’m trusting you
As I bite on though

Taste and See
Eat be free
Bread of Jesus

Why you think
That star it shines
Shining bright
All though the night
Why you think
That ocean’s blue
Did no one
Ever tell you

I think that God made things just the way that they are
To remind people that he is really not so far

Why you think
Why you think

Why you think
That skin your in
Lets you some
Somehow live
Why you think
Those eyes you have
Don’t see that
God’s all is around